Protein Identification and Quantification

Protein sequence identification is a computer intensive task and is often left to dedicated, powerful computers. 

MaxQuant*

Assign a sequence database: Global Parameters > Sequences > Add 

MaxQuant has an internal list of protein modifications that can be edited: Configurations > Modifications

Likewise an internal list of proteases: Configurations > Proteases

Load mass spectrometry data:  Raw data > Load/Load Folder

* S. Tyanova et al., “The MaxQuant computational platform for mass spectrometry-based shotgun proteomics.,” Nat Protoc, vol. 11, no. 12, pp. 2301-2319, 2016

Comments: matthias.wilm@ucd.ie