What you need:
- RNAseq data files (FASTA format, smaller than 5 GBytes each) from human or mouse or
- a Gene expression data table with at least 3 biological repeats for at least 2 conditions (experiment - control).
- A Meta Data table explaining which sample is a biological repeat
- Optionally: a Google account if you want your data to remain private.
3 biological repeats are required to estimate whether the expression difference of a gene between the two states is within the natural variance of expression of this particular gene or not.
Example of a gene expression data table:
N-Starv: non-starvation cells, Starv: starvation cells, 1, 2 3: number of the biological repeats
Example of an Meta Data Table:
Comments: matthias.wilm@ucd.ie
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