macOS Installation

RNAlysis - macOS Installation

- R: A statistical analysis package: Source - Installer (ARM chip) - Installer (Intel chip)

- R Package: DeSeq2 (needs to be installed from the running the R-programme in macOS): Source -

Installation: Run the following code from the R-command line after having started the R programme from the

Applications folder:

if (!require("BiocManager", quietly = TRUE))

install.packages("BiocManager")

BiocManager::install("DESeq2")

- Kallisto : Python script to quantify RNAseq data: Source - Installer: copy the files to the "/Applications/" folder

- RNAlysis : RNAseq data analysis programme: Source - Installer: copy the decompressed RNAlysis file to the

"/Applications/" folder

- FastQC : Sequence quality check programme: Source - Installer: Double click the dmg file and move the FastQC application to the "/Applications/" folder

- Java Runtime Environment: Required to run FastQC: Source - Installer (ARM chip) - Installer (Intel chip) : Double-click the installer and follow instructions

First run of RNAlysis and of FastQC: RNAlysis and FastQC are not signed by a registered Apple Developer. You have to allow the system to run the application:

- "control-click" RNAlysis or FastQC and choose the context menu: Open

- click the Open button

- wait a bit

This is your first test run of RNAlysis