Visualisation

The original tutorial of RNAlysis lists a lot of possibilities to visualise quantitative results. 

The processing steps of tabular data can be collected in Pipelines. Choose the menu Pipelines > New Pipeline.. to create one.

Choose the button-menu command Visualize > Choose a function... > Volcano Plot

Hit the button Add to Pipline

To display only relevant data we can add a filter and split the data into over- and under-expressed genes by adding additional functions to the pipeline.

Choose the button-menu command Filter > Choose a function... > Filter by statistical significance + Add to Pipeline button

Choose the button-menu command Filter > Choose a function... > Split by log2 fold-change direction + Add to Pipeline button

Hit the button Save Pipeline and close the Pipeline window.

Use the command Pipelines > Apply Pipeline > "Name of the Pipeline" to apply the saved pipeline to a data table. 

-> A series of windows will pop up: 

    - Apply the Pipeline in place ?:  No

    - Choose table: Non-Starvation vs Starvation

    - Choose tables to keep: Select all

    - Save the session

Results: 

- A volcano plot was generated (behind the main window)

- Two more tables were generated - one with over- the other with under-expressed genes.

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